新品速遞丨6月上市的經(jīng)嚴(yán)格驗(yàn)證的全新抗體、試劑及試劑盒
STING (E9X7F) Rabbit mAb #90947
對(duì)使用 STING (E9X7F) Rabbit mAb（左圖，紅色）標(biāo)記以及使用 CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399（右圖，綠色）和 DAPI #4083（右圖，藍(lán)色）共標(biāo)記的固定冷凍的小鼠淋巴結(jié)組織進(jìn)行共聚焦免疫熒光分析。
對(duì)使用 STING (E9X7F) Rabbit mAb（上圖，紅色）標(biāo)記以及使用 CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399（下圖，綠色）和 DAPI #4083（下圖，藍(lán)色）共標(biāo)記的固定冷凍的小鼠脾臟組織在低放大倍率（左圖）和高放大倍率（右圖）進(jìn)行共聚焦免疫熒光分析。
對(duì)使用 STING (E9X7F) Rabbit mAb（左圖，紅色）標(biāo)記的固定冷凍的小鼠肺臟組織進(jìn)行共聚焦免疫熒光分析。然后將游離的二抗結(jié)合位點(diǎn)用 Rabbit (DA1E) mAb IgG XP? Isotype Control #3900 封閉，之后用 COL1A1 (E8F4L) XP? Rabbit mAb (Alexa Fluor? 647 Conjugate) #72827（右圖，偽彩青色）和 DAPI #4083（右圖，藍(lán)色）進(jìn)行共標(biāo)記。
使用 STING (E9X7F) Rabbit mAb（綠色）、β-Actin (8H10D10) Mouse mAb #3700（紅色）和 DAPI #4083（藍(lán)色）對(duì) J774A.1 細(xì)胞（左圖，高表達(dá)）和 MBT2 細(xì)胞（右圖，低表達(dá)）進(jìn)行共聚焦免疫熒光分析。
使用 STING (E9X7F) Rabbit mAb（綠色）、β-Actin (8H10D10) Mouse mAb #3700（紅色）和 DAPI #4083（藍(lán)色）對(duì) MOLP-8 細(xì)胞（左圖，陽性）和 A549 細(xì)胞（右圖，陰性）進(jìn)行共聚焦免疫熒光分析。
使用 STING (E9X7F) Rabbit mAb（實(shí)線）或濃度匹配的 Rabbit (DA1E) mAb IgG XP? Isotype Control #3900（虛線）對(duì) A549 細(xì)胞（藍(lán)色，陰性）和 THP-1 細(xì)胞（綠色，陽性）進(jìn)行流式細(xì)胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor? 488 Conjugate) #4412 作為二抗。
使用 STING (E9X7F) Rabbit mAb（實(shí)線）或濃度匹配的 Rabbit (DA1E) mAb IgG XP? Isotype Control #3900（虛線）對(duì) NIH/3T3 細(xì)胞（藍(lán)色，低表達(dá)）和 J774A.1 細(xì)胞（綠色，高表達(dá)）進(jìn)行流式細(xì)胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor? 488 Conjugate) #4412 作為二抗。
左右滑動(dòng)查看更多
干擾素基因刺激蛋白 (STING, TMEM173, MITA) 是一種跨膜接頭蛋白，它是細(xì)胞病原細(xì)胞漿 DNA 的天然免疫應(yīng)答的一個(gè)關(guān)鍵組分 【1,2】。STING 是一種被發(fā)現(xiàn)主要在內(nèi)質(zhì)網(wǎng)中普遍表達(dá)的蛋白 【1】。在細(xì)胞漿 DNA 的刺激下，酶 cGAMP 合成酶 (cGAS) 會(huì)產(chǎn)生第二信使環(huán)-GMP-AMP (cGAMP) 【3,4】。cGAMP 結(jié)合并激活 STING 【3,4】。此外，通過 DDX41 和 IFI16 等核酸感應(yīng)蛋白檢測(cè)細(xì)胞漿 DNA 會(huì)導(dǎo)致 STING 激活 【5,6】。激活后，STING 與 TBK1 轉(zhuǎn)位至核周內(nèi)體，并在 Ser366（小鼠內(nèi)為 Ser365）位點(diǎn)被 ULK1 磷酸化【7, 8】。TBK1 激酶磷酸化并激活 IRF-3 和 NF-κB，從而誘導(dǎo) I 型干擾素和其他免疫應(yīng)答基因 【1,2,7】。
關(guān)鍵詞：天然免疫，自身免疫病，腫瘤免疫
點(diǎn)擊查看更多詳情：STING (E9X7F) Rabbit mAb #90947
|
貨號(hào) |
產(chǎn)品名稱 |
應(yīng)用 |
反應(yīng)性 |
|
Adhesion / ECM |
|||
|
86903T |
WB, IP, IHC-Bond, IHC-P, IF-IC |
H |
|
|
94386SF |
WB, IHC-P, IF-IC, F |
H, M, R |
|
|
10962S |
WB |
H, M, (R) |
|
|
97174SF |
WB, IHC-P, IF-IC, F |
H |
|
|
68534S |
WB, IF-F, IF-IC |
H, M |
|
|
20302T |
WB, IP, IHC-P |
H |
|
|
Apoptosis |
|||
|
74860SF |
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (BSA and Azide Free) |
IHC-P, IF-IC, F |
H, M |
|
95696SF |
Cleaved PARP (Asp214) (D64E10) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, Mk |
|
Cell Cycle / Checkpoint Control |
|||
|
62548SF |
IHC-P, IF-IC, F |
H |
|
|
60480SF |
WB, IHC-P |
H, Mk |
|
|
46565SF |
WB, IHC-P, IF-IC, F |
H |
|
|
41359SF |
Phospho-Rb (Ser807/811) (D20B12) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Mk |
|
Chromatin Regulation / Nuclear Function |
|||
|
52626S |
WB |
H, M, R, Mk |
|
|
73098S |
WB |
H, M, R, Mk |
|
|
85449S |
WB |
H, R, Mk |
|
|
87162S |
WB, IP, ChIP |
H, M, R, Mk |
|
|
65992S |
PSPC1 (E7L8B) Rabbit mAb |
WB, IF-IC |
H, M, (R) |
|
Cytoskeletal Signaling |
|||
|
62114S |
WB |
H, M, R |
|
|
10333S |
WB |
H, M, R |
|
|
56551S |
WB, IP |
H |
|
|
46251S |
WB |
H, M, R |
|
|
69319SF |
WB, IHC-P |
H, M, R |
|
|
Developmental Biology |
|||
|
72528S |
- |
- |
|
|
87548S |
- |
- |
|
|
87179T |
WB, ChIP |
H |
|
|
60770T |
IHC-P, IF-IC |
H |
|
|
42028S |
- |
- |
|
|
25330S |
F |
H, M, R |
|
|
81849S |
F |
H, M, R |
|
|
50850S |
IHC-P |
M |
|
|
Glucose / Energy Metabolism |
0 |
|
|
|
40941S |
WB |
H, M, R |
|
|
Immunology and Inflammation |
|||
|
47359SF |
WB, IHC-Bond, IHC-P |
M |
|
|
32814S |
WB, IHC-Bond, IHC-P |
H |
|
|
32814T |
WB, IHC-Bond, IHC-P |
H |
|
|
53986C |
ELISA |
H |
|
|
49392S |
WB, IP |
H |
|
|
87015S |
- |
- |
|
|
87791SF |
Granzyme B (E5V2L) Rabbit mAb (Mouse Specific) (BSA and Azide Free) |
WB, IHC-Bond, IHC-P, F |
M |
|
29881S |
WB, IP, IF-IC |
H |
|
|
37517S |
WB, IP, IHC-P |
H |
|
|
50384S |
IHC-Bond, IHC-P |
H |
|
|
33469S |
IHC-Bond, IHC-P |
H |
|
|
54059S |
- |
- |
|
|
95742SF |
WB, IHC-Bond, IHC-P, IF-F, F |
M |
|
|
98018S |
- |
- |
|
|
64063S |
- |
- |
|
|
79627S |
- |
- |
|
|
84087S |
- |
- |
|
|
74906S |
WB |
H |
|
|
60847SF |
IHC-Bond, IHC-P |
H |
|
|
39356SF |
WB, IHC-P |
H |
|
|
24224SF |
PD-L1 (Extracellular Domain Specific) (E1J2J?) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P |
H |
|
37908S |
WB, IHC-P |
H |
|
|
37908T |
WB, IHC-P |
H |
|
|
58551S |
WB, F |
H |
|
|
88845SF |
Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M |
|
73533SF |
Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-Bond, IHC-P, IF-IC, F |
H, M, R, Mk |
|
51879SF |
Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, F |
H, M |
|
90947S |
IF-F, IF-IC, F |
H, M |
|
|
16769S |
- |
- |
|
|
24095S |
- |
- |
|
|
87804S |
WB |
H |
|
|
Infectious Diseases |
|||
|
19595S |
WB |
Vir |
|
|
93220S |
WB |
Vir |
|
|
MAP Kinase Signaling |
|||
|
77955SF |
Phospho-c-Jun (Ser73) (D47G9) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-F, IF-IC, F |
H, M, R, Mk, Pg |
|
62979SF |
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Mk, Mi, Pg, Sc |
|
45899SF |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Hm, Mk, Mi, Dm, Z, B, Dg, Pg, Sc |
|
55773SF |
Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC |
H, Mk |
|
58328SF |
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P |
H, M, R, Dm, Sc |
|
Metabolism |
|||
|
63536S |
WB |
H, M, R |
|
|
49381S |
WB |
H, M, R |
|
|
84476S |
WB, IP |
H |
|
|
Motif Antibodies |
|||
|
68517S |
- |
- |
|
|
Neuroscience |
|||
|
39561SF |
Phospho-CREB (Ser133) (87G3) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-F, IF-IC, F |
H, M, R |
|
58595S |
WB, IF-F, IF-IC |
H, M, R |
|
|
57187S |
WB |
M |
|
|
PI3K / Akt Signaling |
|||
|
80722SF |
Phospho-Akt (Thr308) (D25E6) XP? Rabbit mAb (BSA and Azide Free) |
WB, IF-IC, F |
H, M, R, Mk |
|
54932SF |
Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) (BSA and Azide Free) |
IHC-P |
H |
|
Protein Folding and Trafficking |
|||
|
72312S |
WB |
H, M, R |
|
|
RTK |
|||
|
64191SF |
Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P |
H, M |
|
Translational Control |
|||
|
39788SF |
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Mk, Dm |
|
16300S |
WB, ChIP |
H, M, R, Mk |
|
|
49253S |
WB, IP |
H, M, R, Mk |
|
|
88145S |
WB |
H, M, R, Mk |
|
|
41645S |
WB |
H |
|
|
36103S |
WB, IP, IF-F, IF-IC |
H, M, R, Mk |
|
|
59753S |
WB, IP |
H, M, R, Mk |
|
|
81736SF |
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Mk, Mi, Sc |
|
50837S |
WB, IP |
H |
|
|
Related Products |
|||
|
10612SF |
WB, IHC-P, IF-IC, F |
All |
|
|
60395S |
Mouse Progenitor Exhausted CD8+ T Cell Markers Flow Cytometry Panel |
F |
M |
上下滑動(dòng)查看更多
如需購買產(chǎn)品、咨詢價(jià)格，請(qǐng)點(diǎn)擊下方“經(jīng)銷商查詢”，聯(lián)系您所在區(qū)域的CST授權(quán)經(jīng)銷商，確保正品。
【參考文獻(xiàn)】
1.Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
2. Zhong, B. et al. (2008) Immunity 29, 538-50.
3. Sun, L. et al. (2013) Science 339, 786-91.
4. Wu, J. et al. (2013) Science 339, 826-30
5. Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.
6. Unterholzner, L. et al. (2010) Nat Immunol 11, 997-1004.
7. Ishikawa, H. et al. (2009) Nature 461, 788-92.
8. Konno, H. et al. (2013) Cell 155, 688-98.
© 2022 Cell Signaling Technology, Inc.版權(quán)所有。Cell Signaling Technology、CST、XP、PTMScan均是Cell Signaling Technology, Inc.的商標(biāo)，所有其他商標(biāo)均為其各自所有者的財(cái)產(chǎn)。
北京百靈克生物科技有限責(zé)任公司
北京市海淀區(qū)永定路88號(hào)長銀大廈9C10
產(chǎn)品咨詢電話：010-58895880/58896088
官方網(wǎng)站：m.qlcapital.com.cn
官方微信：百靈克生物Biolink
- 上一篇：黑色素瘤：異常的信號(hào)轉(zhuǎn)導(dǎo)通路和治療方法丨探秘癌癥（二） 2022/6/10
- 下一篇：春已至，萬物始，TOYOBO邀您邂逅春夏時(shí)光！ 2022/6/9
